![]() ![]() This modification increases Tm of RNA:RNA duplexes but results in only small changes in RNA:DNA stability. Oligonucleotides can be directly synthesized that contain 2'-O-Methyl RNA. View product details for 2-MethoxyEthoxy A:Ģ'-O-Methyl RNA is a naturally occurring modification of RNA found in tRNA and other small RNAs that arises as a post-transcriptional modification. For more information on antisense technologies and strategies please see: Antisense oligonucleotides. ![]() Compared to standard RNA bases 2’-MOE bases offer increased resistance to nuclease degradation, reduced toxicity, and increased affinity for binding to complimentary RNA. You can include up to 20 Affinity Plus bases in Affinity Plus DNA & RNA Oligonucleotides, and up to 6 Affinity Plus bases in Affinity Plus qPCR Probes.Ģ’-MOE bases are often used for antisense oligos (ASO), aptamers, and siRNA. When ordering, denote Affinity Plus bases in your sequence using +A, +G, +C, +T. Learn more about the benefits of including Affinity Plus bases in your oligo and probe sequences by visiting our Locked nucleic acid technology page. These bases also confer significant in vitro and in vivo nuclease resistance. Addition of locked nucleic acid monomers help to increase and can be used to fine-tune sequence melting temperature (T m). Locked nucleic acids-Affinity Plus modified basesĪffinity Plus modified bases are locked nucleic acids. Target Capture Probe Design & Ordering Tool.Library Concentration Conversion Calculator.Alt-R Predesigned Cas9 crRNA Selection Tool. ![]()
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